Purification and reconstitution of the calcium release channel from skeletal muscle
- 1 January 1988
- journal article
- Published by Springer Nature in Nature
- Vol. 331 (6154) , 315-319
- https://doi.org/10.1038/331315a0
Abstract
The calcium release channel from rabbit muscle sarcoplasmic reticulum (SR) has been purified and reconstituted as a functional unit in lipid bilayers. Electron microscopy reveals the four-leaf clover structure previously described for the 'feet' that span the transverse tubule (T)-SR junction. Ca2+ release from the SR induced by T-system depolarization during excitation-contraction coupling in muscle may thus be effected through a direct association of the T-system with SR Ca2+-release channels.Keywords
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