Guanosine Triphosphate Dependent Enzymic Binding of Aminoacyl Transfer Ribonucleic Acid to Yeast Ribosomes
Open Access
- 1 December 1968
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 7 (1) , 111-118
- https://doi.org/10.1111/j.1432-1033.1968.tb19581.x
Abstract
The binding of phenylalanyl‐tRNA to purified yeast ribosomes at low magnesium concentrations is dependent upon one of the yeast supernatant transfer factors (factor A). This reaction requires GTP. The GTP analogue guanylyl methylene diphosphonate cannot be substituted for GTP but it acts as a good inhibitor. After incubation of the ribosomes with phenylalanyl‐tRNA, factor A and GTP practically no compound other than phenylalanyl‐tRNA itself is found linked to the ribosomes.In addition to this enzymic type of binding, phenylalanyl‐tRNA is also bound to yeast ribosomes in the absence of factor A and GTP provided that the magnesium concentration is increased to about 20 mM. This non‐enzymic binding is effectively inhibited by deacylated tRNA and this inhibition is prevented if factor A and GTP are included in the incubation mixtures.The binding of N‐acetylphenylalanyl‐tRNA to yeast ribosomes also requires GTP, and is dependent upon factor(s) other than the amino acid transfer factors, which are bound to the ribosomes.Keywords
This publication has 32 references indexed in Scilit:
- The binding of aminoacyl sRNA and GTP to transferase IBiochemical and Biophysical Research Communications, 1967
- Properties of ribosomal binding sites for phenylalanyl-transfer ribonucleic acidJournal of Molecular Biology, 1967
- GTP interaction with a protein synthesis initiation factor preparation from EscherichiacoliBiochemical and Biophysical Research Communications, 1967
- Catalysis of peptide bond formation by 50 s ribosomal subunits from Escherichia coliJournal of Molecular Biology, 1967
- Regulatory mechanisms and protein synthesis X. Codon recognition on 30 s ribosomesJournal of Molecular Biology, 1966
- A competitive inhibitor of the GTP reaction in protein synthesisJournal of Molecular Biology, 1966
- The requirements for specific sRNA binding by ribosomesJournal of Molecular Biology, 1966
- Reversal by GTP of soluble RNA inhibition of polyphenylalanine synthesisBiochemical and Biophysical Research Communications, 1966
- The puromycin reaction and its relation to protein synthesisJournal of Molecular Biology, 1964
- A ribosome-bound intermediate in polypeptide synthesisBiochemical and Biophysical Research Communications, 1963