Heterogeneous Nuclear RNA from Lymphocytes of Chronic Lymphocytic Leukaemia: Adenylate‐Rich and Double‐Stranded Regions

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Abstract
Rapidly labelled high molecular weight nuclear RNA from lymphocytes of chronic lymphocytic leukaemia was analysed for ribonuclease‐stable adenylate‐rich and double‐stranded regions. The polyadenylate content corresponds to 0.4‐0.5 % and the content of double‐stranded sequences to 2–4 % of the total nucleotides. Partial association of polyadenylate segments with double‐stranded regions was found by comparative analysis of (3H)‐adenosine and (3H)‐uridine labelled ribonuclease‐stable RNA before and after thermal denaturation. Comparison with normal lymphocytes shows lower proportions of polyadenylate‐containing RNA binding to poly(U)‐Sepharose in leukaemia cells than in normals. Partial degradation of rapidly labelled high molecular weight RNA was found in leukaemia cases with low white cell counts.