Further Studies of Radiaton-Induced Interphase Death of Cultured Mammalian Cells

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Abstract
Interphase death of cells in the stationary phase is compared with that seen in irradiated cells in the exponential phase of growth. Cells in the stationary phase seem slightly more radioresistant. Death of cells in stationary phase is accelerated by presence of DNP or puromycin while irradiated exponential growth phase cells show accelerated cell death only with dinitrophenol. Death of stationary phase cells irradiated with relatively low doses (0.5 and 5 rads) is unchanged from spontaneous death of nonirradiated stationary phase cells while exponential phase cells die in a two-step fashion after irradiation at these doses. Irradiated cells which were cooled to 26°C did not die until rewarmed to 37°C. Decreased serum concentration in the growth medium of stationary phase cells slowed the death process. These findings suggest over-all disorganization of cellular structures as an explanation of radiation-induced interphase death.

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