Purification of glycollate oxidase from greening cucumber cotyledons
- 1 January 1982
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 156 (6) , 566-571
- https://doi.org/10.1007/bf00392782
Abstract
Glycollate oxidase (glycollate: oxygen oxidoreductase, EC 1.1.3.1) was purified to apparent homogeneity from crude extracts of greening cucumber cotyledons (Cucumis sat vus). Molecular sieving and chromatofocusing resulted in 700-fold purification and specific activity of 1 μkat mg-1 protein. The enzyme exhibited a Mr of 180,000, or 700,000, respectively, and is a tetramer or 16-mer made of identical subunits of Mr 43,000. Monospecific antibodies were raised against the homogeneous protein.Keywords
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