Automated Amino Acid Analysis with Sensitive Fluorescence Detection

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Abstract
The fluorigenic reagent o-phthalaldehyde-mercaptoethanol gives good reproducibility with a very stable baseline when applied to the automated analysis of amino acids at the nanomole level. Determination of even smaller quantities is possible; basic amino acids are preferably eluted separately at constant pH (e.g., pH 6.0); this eliminates the baseline irregularities that occur with single column systems at high sensitivity settings. The reagent gives excellent results in the assay of small quantities of biological fluids such as blood plasma.