Establishment of an Epstein-Barr Virus-Determined Nuclear Antigen-Negative Human B-Cell Line From Acute Lymphoblastic Leukemia: Brief Communication2

Abstract
A human B-cell line designated as BALL-1 was established from the peripheral blood of a patient with acute Iymphoblastic leukemia (ALL). Neither Epstein-Barr virus (EBV) particles nor EBV-determined nuclear antigen (EBNA) was detectable. The morphologic and growth characteristics were clearly distinct from those of numerous EBV-positive Iymphoblastoid cell lines previously reported. BALL-1 cells probably originated from the donor's leukemia cells as judged from their cytogenetic, morphologic, and surface features. The BALL-1 line was the first EBNA-negative B-cell line established from ALL.

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