A New Electrochemical Approach for thein VitroInvestigation of Damage in Native DNA by Small γ-radiation Doses
- 1 January 1982
- journal article
- research article
- Published by Taylor & Francis in International Journal of Radiation Biology
- Vol. 42 (4) , 407-415
- https://doi.org/10.1080/09553008214551331
Abstract
Voltammetry provides a new method to assess γ-radiation damage to native DNA in vitro after low doses of irradiation. Using single sweep voltammetry at a stationary mercury electrode a substantial labilization of the double helical conformation of γ-irradiated DNA was found. Up to 40 Gy a linear dose-effect relationship exists reflected for the number of strand breaks and the number of destabilized base pairs. A considerable difference in the frequency of formation of the two kinds of radiation damage is seen in the ratio of their G-values. Base pair labilization occurs depending on aeration conditions 200 to 300 times more frequently than the formation of a single strand break.This publication has 22 references indexed in Scilit:
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