Sequencing of oligonucleotides using high performance liquid chromatography and electrospray mass spectrometry
- 1 January 1995
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 9 (10) , 897-901
- https://doi.org/10.1002/rcm.1290091007
Abstract
We report the sequence analysis of an oligodeoxyribonucleotide by electrospray mass spectrometry (ES‐MS). For full sequence analysis of the oligomer, d(CGTAGCTACG), an enzyme digestion step followed by off‐line high performance liquid chromatography was used. For ES‐MS analysis, introducing the sample in an imidazole buffer with 70% acetonitrile increased the electrospray ion currents and helped reduce sodium‐adduct ion peaks.Keywords
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