Regulation of the production of granulocyte-macrophage colony-stimulating factor by macrophage-like tumour cell lines
- 28 January 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 180 (2) , 271-274
- https://doi.org/10.1016/0014-5793(85)81084-x
Abstract
Macrophage tumour cell lines (PU5-1.8, P388D1) produced detectable granulocyte macrophage colonystimulating (CSF-2) activity measured using a factor-dependent cell line FDC-P1. The production ofCSF-2 was enhanced by endotoxin and inhibited by serum, and correlated inversely with [3H]TdR incorporation. mRNA isolated from PU5-1.8 or P388D1 cells initiated CSF-2 production when injected into Xenopus laevis oocytes. The specific activity in this assay was unaltered in mRNA isolated from endotoxin-treated cells. The results suggest that endotoxin acts at a post-transcriptional levelKeywords
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