A non-barbital buffer for immunoelectrophoresis and zone electrophoresis in agarose gels.

Open Access

1 October 1978

journal article
research article
Published by Oxford University Press (OUP) in Clinical Chemistry

Vol. 24 (10) , 1825-1827
https://doi.org/10.1093/clinchem/24.10.1825

Abstract

We describe buffer for both immunoelectrophoresis and zone electrophoresis procedures in agarose gels, Tricine [N-tris(hydroxymethyl)methylglycine] and Tris [2-amino-2-(hydroxymethyl)-1,3-propanediol] being the main components. This buffer eliminates the procurement cost and inventory control associated with formulation of barbital buffers. We compare it with other common buffer formulations.