Bioimaging of the unbalanced expression of microRNA9 and microRNA9* during the neuronal differentiation of P19 cells

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Abstract
Generally, the 3′‐end of the duplex microRNA (miR) precursor (pre‐miR) is known to be stable in vivo and serve as a mature form of miR. However, both the 3′‐end (miR9) and 5′‐end (miR9*) of a brain‐specific miR9 have been shown to function biologically in brain development. In this study, real‐time PCR analysis and in vitro/in vivo bioluminescent imaging demonstrated that the upstream region of a primary miR9‐1 (pri‐miR9‐1) can be used to monitor the highly expressed pattern of endogenous pri‐miR9‐1 during neurogenesis, and that the Luciferase reporter gene can image the unequal expression patterns of miR9 and miR9* seen during the neuronal differentiation of P19 cells. This demonstrates that our bioimaging system can be used to study the participation of miRs in the regulation of neuronal differentiation.