Inhibition of the Hammerhead Ribozyme Cleavage Reaction by Site-Specific Binding of Tb(III)
- 2 January 1998
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 279 (5347) , 81-84
- https://doi.org/10.1126/science.279.5347.81
Abstract
Terbium(III) [Tb(III)] was shown to inhibit the hammerhead ribozyme by competing with a single magnesium(II) ion. X-ray crystallography revealed that the Tb(III) ion binds to a site adjacent to an essential guanosine in the catalytic core of the ribozyme, approximately 10 angstroms from the cleavage site. Synthetic modifications near this binding site yielded an RNA substrate that was resistant to Tb(III) binding and capable of being cleaved, even in the presence of up to 20 micromolar Tb(III). It is suggested that the magnesium(II) ion thought to bind at this site may act as a switch, affecting the conformational changes required to achieve the transition state.Keywords
This publication has 31 references indexed in Scilit:
- Capturing the Structure of a Catalytic RNA Intermediate: The Hammerhead RibozymeScience, 1996
- Metal-binding sites in the major groove of a large ribozyme domainStructure, 1996
- Catalytic Activity and Stability of Hammerhead Ribozymes Containing 2′-Acetamido-2′-DeoxyribonucleosidesBiochemical and Biophysical Research Communications, 1995
- Three-dimensional structure of a hammerhead ribozymeNature, 1994
- The CCP4 suite: programs for protein crystallographyActa Crystallographica Section D-Biological Crystallography, 1994
- A Kinetic and Thermodynamic Framework for the Hammerhead Ribozyme ReactionBiochemistry, 1994
- Effects of phosphorothioate and 2-amino groups in hammerhead ribozymes on cleavage rates and magnesium bindingBiochemistry, 1991
- The automated chemical synthesis of long oligoribuncleotides using 2'-O-silylated ribonucleoside 3'-O-phosphoramidites on a controlled-pore glass support: synthesis of a 43-nucleotide sequence similar to the 3'-half molecule of an Escherichia coli formylmethionine tRNAJournal of the American Chemical Society, 1987
- A crystallographic study of metal-binding to yeast phenylalanine transfer RNAJournal of Molecular Biology, 1977
- Europium as a fluorescent probe of transfer RNA structureBiochemistry, 1975