Characterization of a Nonspecifically Aphid-Transmitted CA-RPV Isolate of Barley Yellow Dwarf Virus

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Abstract
An isolate (CA-RPV-1) of barley yellow dwarf virus (BYDV) recovered from barley (Hordeum vulgare) in Davis, California [USA] was transmitted to oats (Avena sativa) in a nonspecific manner by New York (NY) clones of Rhopalosiphum padi, Sitobion avenae, and Schizaphis graminum and by a California (CA) clone of Sitobion avenae. The oat plants showed characteristic BYDV symptoms. In double-antibody-sandwich and indirect monoclonal antibody ELISA, CA-RPV-1 reacted strongly to antisera made to NY-RPV and in some tests to a lesser degree to antisera made to NY-PAV or NY-MAV. CA-RPV-1 was serologically indistinguishable from NY-RPV, regardless of which aphid species had transmitted it. The dsRNA banding pattern from plants infected by CA-RPV-1 was identical to that of NY-RPV.CA-RPV-1 did not react with recombinant cDNA plasmids made to NY-RPV, NY-PAV, MY-MAV, Australian PAV isolates of BYDV, or beet western yellows virus in nucleic acid dot hybridizations. Nonspecific transmission was found in 26% of the CA-RPV field isolates tested.