Effect of Insulin on LHRH Release by Perifused Hypothalamic Fragments

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Abstract
Insulin-deficient states are associated with an impaired function of the hypothalamic-pituitary-gonadal axis, but the mechanisms underlying hypothalamic alterations in experimental diabetes are still unknown. We investigated the effect of glucose concentrations, in the presence and absence of insulin, on LHRH release from perifused hypothalamic fragments from female adult ovariectomized rats. Glucose and insulin were added to the perifusion medium (Earle’s, pH 7.4, gassed with 95% O2/5% CO2, flow rate 50 µl/min). When glucose was absent (in the presence of insulin 10 mU/1), LHRH release was reduced, peak levels being

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