Competitive inhibition of colchicine binding to tubulin by microtubule-associated proteins.
- 1 January 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (1) , 86-90
- https://doi.org/10.1073/pnas.76.1.86
Abstract
Microtubule-associated proteins (MAP) promote tubulin polymerization, but colchicine inhibits this process. MAP inhibit colchicine binding to tubulin in a competitive manner. Attempts were made to identify which of the MAP fraction(s) was responsible: both tau protein (a thermostable molecule with a MW of .apprx. 70,000) and a high MW fraction completed with colchicine. In contrast, Mg2+, which also induces microtubule assembly in vitro, had no effect on colchicine binding to tubulin.Keywords
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