A modified wire-loop method for quantitative electron microscopic radioautography
- 1 January 1978
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 57 (1) , 93-96
- https://doi.org/10.1007/bf00507359
Abstract
Summary A simplified method of emulsion coating and development for quantitative electron microscopic radioautography without any special instrument is introduced. Seven grids carrying ultrathin sections are attached to a glass block with double-coated Scotch tape. They are coated with a wire-loop, 2.5 cm in diameter. Ten glass blocks are then stuck on a slide, exposed, developed, fixed and stained simultaneously. Employing this method, the emulsion coating and the following procedures are easily accomplished under an identical condition.Keywords
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