The Isolation of Vacuoles from Candida Utilis
- 1 January 1973
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 3 (6) , 553-561
- https://doi.org/10.1080/00327487308061537
Abstract
Vacuoles containing S-adenosylmethionine have been isolated from the yeast, Candida utilis, by the osmotic lysis of its spheroplasts and differential centrifugation. Culture in the presence of L-methionine and harvest in the stationary phase contributed to the ease of isolation and stability of the vacuoles. The vacuoles showed retentiveness for the sulfonium compound and responded to the vital-staining reaction with Neutral Red. Ultraviolet micrography indicated the homogeneity of the vacuolar preparation.Keywords
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