Inducible and Constitutive Formation of Fructanase in Batch and Continuous Cultures of Streptococcus mutans
- 1 July 1985
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 131 (7) , 1625-1633
- https://doi.org/10.1099/00221287-131-7-1625
Abstract
The production of extracellular .beta.-D-fructanase by several strains of S. mutans was studied in continuous culture. When glucose was the limiting nutrient, S. mutans K1-R and OMZ176 accumulated fructanase to maximum levels at low growth rates (dilution rate 0.05-0.10 h-1), due to the longer residence times of the bacteria in the culture vessel under these conditions. Extracellular fructanase activity was greater than was previously reported for batch cultures. The rate of fructanase production for both S. mutans strains K1-R and OMZ176 increased with increasing growth rate when glucose was limiting. Under conditions of glucose sufficiency, the rate of fructanase production was always lower than in cultures where glucose was limiting, irrespective of the growth rate. Cultures of S. mutans Ingbritt (serotype c) grown with sorbitol- or glucose limitation synthesized fructanase at a very low basal rate. When fructose was the limiting carbohydrate the enzyme was induced with a maximum rate of production occurring at a dilution rate of 0.40 h-1. Strains of S. mutans from other serotypes (a,d,d/g) were either not affected by changing the limiting sugar from glucose to fructose or else fructanase activity was slightly decreased in the fructose-limited medium. Fructanases from various strains of S. mutans readily hydrolyzed (2 .fwdarw. 6)-.beta.-D-fructans, but all possessed the ability to hydrolyze (2 .fwdarw. 1)-.beta.-D-fructans to varying degrees.This publication has 10 references indexed in Scilit:
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