USE OF ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR DETECTION OF ANTIBODIES TO BRUCELLA-OVIS IN SHEEP - FIELD TRIAL

  • 1 January 1985
    • journal article
    • research article
    • Vol. 46  (8) , 1642-1646
Abstract
An ELISA, using an autoclave-extracted soluble antigen, for the detection of serum antibodies to B. ovis in sheep was developed. The test seemed to be both sensitive and specific, on the basis of the control groups studied. The antigen showed no deterioration in prepared plates stored at -70.degree. C for up to 1 yr. The ELISA was used in conjunction with palpation of rams for epididymal lesions as a means to detect and control B. ovis infection in a naturally infected flock. All rams were evaluated by the ELISA. At the time that the 1st blood sample was obtained, all positive and suspicious reactors were removed. With subsequent blood sample collections, only the positive reactors were removed. B. ovis was not isolated from any rams during the following year, and none of the mature breeding rams developed epididymal lesions.

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