The isolation of mutants ofAspergillus flavusandA.Parasiticuswith altered aflatoxin producing ability

Abstract
Mutations affecting the production of aflatoxin might be useful in studying its biosynthesis, and in understanding the wide variation in aflatoxigenicity of fungal strains isolated from nature. Two methods of isolating mutants of Aspergillus in which aflatoxin production is attenuated or lost are presented. In one method mutants are selected on the basis of altered fluorescence under ultraviolet light. The second method utilizes a mutant strain which produces aflatoxin and an orange-red mycelial pigment not detected in the wild type. Unpigmented mutants derived from this strain exhibit lowered or lost aflatoxigenicity.

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