Role of a serum phospholipase A1in the phosphatidylserine‐induced T cell inhibition
- 18 January 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 316 (1) , 1-4
- https://doi.org/10.1016/0014-5793(93)81724-e
Abstract
We have previously shown that unsaturated phosphatidylserines inhibit mitogen‐induced T cell activation. We now report that the inhibitory action requires a protein present in bovine and human serum. Partial purification and phospholipase assay show that this protein has phospholipase A activity on phosphatidylserine but not phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol. In short incubations (1–3 h) 2‐acyl lysophosphatidylserine is produced but in longer incubations thecis‐unsaturated fatty acid is also released. Experiments on peripheral blood mononuclear cells indicate that the unsaturated fatty acid becomes the main responsible for the PS‐induced inhibition and that 2‐acyl lysophosphatidylserine enhances the inhibitory effect of fatty acid.Keywords
This publication has 17 references indexed in Scilit:
- Inhibition of DNA synthesis in peripheral blood mononuclear cells treated with phosphatidylserines containing unsaturated acyl chainsImmunopharmacology, 1992
- Inhibition of phospholipase A2 by heparinBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1990
- Signalling mechanism in the lysophosphatidylserine-induced activation of mouse mast cellsBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1990
- Lysophosphatidylserine-dependent interaction between rat leukocytes and mast cellsBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1987
- Immunoregulation by low density lipoproteins in man. Inhibition of mitogen-induced T lymphocyte proliferation by interference with transferrin metabolism.Journal of Clinical Investigation, 1984
- Isolation and characterization of human plasma lipid transfer proteins.Arteriosclerosis: An Official Journal of the American Heart Association, Inc., 1984
- Intracellular phospholipases ABiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1980
- The formation of phosphatidylinositol by acylation of 2-acyl-sn-glycero-3-phosphorylinositol in rat liver microsomesLipids, 1979
- Phospholipid hydrolysis by phospholipases A1 and A2 in plasma membranes and microsomes of rat liverBiochimica et Biophysica Acta (BBA) - Biomembranes, 1973