Further Studies of the Effect of Dilution Rate on the Fertility of Bull Semen Used for Artificial Insemination

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Abstract
In a study involving 3,296 inseminations, dilution rates of 1 part of semen to 8, 12, 16, 24 and 50 parts of yolk-citrate diluter were compared. The expt. was designed so as to eliminate all arbitrary selection of the semen to be used at any dilution rate. On the average, the following number of spermatozoa were contained in the 1 ml. of diluted semen used for each insemination: 150, 104, 80, 54, and 26 millions. No statistically significant differences were found in fertility of the semen for the 5 dilution rates compared. The semen diluted at the highest rate was not used as efficiently as the semen diluted at the lower rates, but, on the average, seemed to maintain its fertility as well for 4 days after collection.

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