MICROSPECTROPHOTOMETRIC STUDIES ON VISUAL PIGMENT IN INTACT RETINA OF GOLDFISH, CARASSIUS-AURATUS (LINN)

  • 1 January 1978
    • journal article
    • research article
    • Vol. 37  (3) , 143-156
Abstract
The visual pigments in intact retinas of the common goldfish were examined by microspectrophotometry. Difference spectra free of photoproduct contamination and absorbance maxima at .lambda. 527 nm .+-. 2 nm. The average pigment density, about 0.32, was almost invariant with regard to retinal location and specimen size. Traces of a photoproduct absorbing maximally at .lambda. 480-490 nm were found. The normalized absorbance curve deviated from standard templates. This may be an effect of crosscoupling of energy among photoreceptors. Known action spectra showed a red shift relative to the absorbance spectra measured.