In situ Degradation of the Protein Chain of Potato Virus X at the N- and C-termini
- 1 February 1978
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 38 (2) , 329-337
- https://doi.org/10.1099/0022-1317-38-2-329
Abstract
The amino acid composition, behavior in SDS[sodium dodecyl sulfate]-polyacrylamide gel electrophoresis and electrophoretic patterns of CNBr peptides were studied for the protein subunits of different preparations of potato virus X (PVX). The protein subunits of PVX can be partially degraded in the intact virus at the N[amino]-terminus by reducing agent-dependent proteases in crude plant sap and by trypsin [EC 3.4.4.4], and at the C[carboxyl]-terminus by reducing agent-independent proteases occurring in some virus preparations.This publication has 1 reference indexed in Scilit:
- Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresisArchives of Biochemistry and Biophysics, 1968