Detection by radioimmunoassay of igm class antibody to hepatitis b core antigen: a comparison of two methods

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Abstract
A solid‐phase radioimmunoassay (RIA) for IgM anti‐HBc is described. The assay (anti‐m̈ RIA) is based on the adsorption of IgM to a tube coated with sheep antibody to the human IgM m̈‐chain. The adsorbed immunoglobulin is assayed for anti‐HBc activity. Positive reactions in the test are shown to be due to IgM antibody, and a confirmation blocking test is described. Reactivity of test sera can be quantitated by comparison with standard sera containing known levels of IgM anti‐HBc.Sera assayed for IgM anti‐HBc by both the anti‐m̈ RIA and a method employing serum fractionation and competitive RIA gave similar results. Rheumatoid factor (RF) alone did not react in the anti‐m̈ RIA, but reactivity could be generated in the presence of RF and preformed aggregates of IgG anti‐HBc.