Uridine Diphosphate-4-Keto-Glucose, an Intermediate in the Uridine Diphosphate-Galactose-4-Epimerase Reaction
- 1 November 1971
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 68 (11) , 2660-2663
- https://doi.org/10.1073/pnas.68.11.2660
Abstract
When UDP-galactose 4-epimerase (EC 5.1.3.2) from Escherichia coli is incubated with UDP-galactose, then reduced with NaB(3)H(4), label is found in UDP-glucose and UDP-galactose. Enzymatic and chemical degradation demonstrates that the label is bound to carbon 4 of the glycosyl moieties. These results provide direct evidence for the existence of UDP-4-keto-glucose as an enzyme-bound intermediate in the epimerization reaction, and they exclude the formation of a 3-keto intermediate.Keywords
This publication has 8 references indexed in Scilit:
- On the mechanism of the UDP-D-glucose-4′-epimerase evidence for a 3-keto-hexose intermediateBiochemical and Biophysical Research Communications, 1971
- Uridine diphosphoglucose 4-epimerase: Kinetic isotope effectBiochimica et Biophysica Acta (BBA) - Enzymology, 1970
- Biosynthesis of uridine diphosphate D-xylose. IV. Mechanism of action of uridine diphosphoglucuronate carboxy-lyase.1970
- Biosynthesis of Uridine Diphosphate D-Xylose. II. Uridine Diphosphate D-Glucuronate Carboxy-lyase of Cryptococcus laurentii*Biochemistry, 1966
- Specific activity determination of radioactive formic acidAnalytical Biochemistry, 1965
- ENZYMES OF GALACTOSE OPERON IN ESCHERICHIA COLI .I. PURIFICATION + CHARACTERIZATION OF URIDINE DIPHOSPHOGALACTOSE 4-EPIMERASE1964
- THE ENZYMIC INTERCONVERSION OF URIDINE DIPHOSPHOGALACTOSE AND URIDINE DIPHOSPHOGLUCOSEJournal of Biological Chemistry, 1957
- ENZYMATIC FORMATION OF URIDINE DIPHOSPHOGLUCURONIC ACIDJournal of Biological Chemistry, 1957