Purification and Characterization of a Cation-stimulated Adenosine Triphosphatase from Corn Roots
Open Access
- 1 August 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 62 (2) , 165-172
- https://doi.org/10.1104/pp.62.2.165
Abstract
A membrane-bound, monovalent cation-stimulated ATPase from Zea mays roots has been purified to a single band on sodium dodecyl sulfate gel electrophoresis. Microsomal preparations with K+ -stimulated ATPase activity were extracted with 1 m NaClO4, and the solubilized enzyme was purified by chromatography on columns of n-hexyl-Sepharose, DEAE-cellulose, and Sephadex G-100 Superfine. A 500-fold purification over the activity present in the microsomes was obtained. The K+ -stimulated activity shows positive cooperativity with increasing KCl concentrations. The purified enzyme shows K+ -stimulated activity with ATP, GTP, UTP, CTP, ADP, α + β-glycerophosphate, p-nitrophenyl phosphate, and pyrophosphate as substrates. Under most conditions ATP is the best substrate. Although dicyclohexyl carbodiimide and Ca2+ inhibit and alkylguanidines stimulate the K+ -ATPase while bound to microsomes, they have no effect on the purified enzyme.This publication has 19 references indexed in Scilit:
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