An Assay for Human Myoglobin Using the Technique of Membrane Immunoassay with Light-Sensitive Nylon Supports
- 1 December 1994
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 27 (15) , 2961-2971
- https://doi.org/10.1080/00032719408000304
Abstract
A method for rapid determination of human myoglobin was developed. The method is based on the technique of membrane immunoassay with light-sensitive nylon membranes for covalent immobilization of antibodies. The kinetic of binding of myoglobin with immobilized antibodies was studied. The pumping of reagents through the pores of a membrane allows to reduce the total assay time to 40 min. The sensitivity of membrane assay of myoglobin was 20 ng/ml. A semi-quantitative method with visual detection also was proposed. It allows to distinguish various states of myocardial infarction pathology.Keywords
This publication has 5 references indexed in Scilit:
- Detection System for Membrane Immunoassay Based on the Trapping of a Highly Colored Intermediate of the Peroxidase ReactionAnalytical Biochemistry, 1993
- Field evaluation of alternative HIV testing strategy with a rapid immunobinding assay and an agglutination assayThe Lancet, 1991
- ‘Key paper’ for covalent binding of proteins and its usesJournal of Immunological Methods, 1986
- ImmunoConcentration--a new format for solid-phase immunoassays.Clinical Chemistry, 1985
- The role of serum myoglobin in the detection and measurement of myocardial infarctionClinica Chimica Acta; International Journal of Clinical Chemistry, 1980