Do metal ions promote the re-activation of the 2,3-bisphosphoglycerate-independent phosphoglycerate mutases?
Open Access
- 14 May 1988
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 252 (1) , 111-117
- https://doi.org/10.1042/bj2520111
Abstract
It has been reported [Smith, McWilliams & Hass (1986) Biochem. Biophys. Res. Commun. 136, 336-340] that addition of certain metal ions, notably Co2+ and Mn2+, promoted the refolding of denatured phosphoglycerate mutase from wheat germ. We have re-investigated these experiments and have shown that, when precautions are taken to avoid artefacts in the assay system, the metal ions do not promote any re-activation of the denatured wheat-germ or Aspergillus nidulans enzymes. An alternative explanation is offered for the observations of Smith et al. (1986).This publication has 22 references indexed in Scilit:
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