Selective triggering of B cell subpopulations by mitogens

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Abstract
We studied the pattern of responsiveness of normal spleen cells to three different B cell mitogens: dextran sulfate (DS), lipopolysaccharide (LPS) and purified protein derivative of tuberculin (PPD). Activation by DS results in extensive proliferation of cells, most of which are not high‐rate antibody‐secreting cells and can be reactivated. PPD activates high‐rate antibody synthesis in cells which are more restricted in mitotic capacity. LPS seems to activate both of these subsets of B cells.LPS exhibits strong mitogenicity in vivo and a large fraction of LPS‐activated cells are high‐rate antibody‐secreting end cells.It is suggested that these distinct subsets of B cells include cells at different stages of differentiation.