A Simple, Rapid Method for Enumerating Total Viable and Metabolically Active Bacteria in Groundwater
- 1 June 1988
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 54 (6) , 1630-1631
- https://doi.org/10.1128/aem.54.6.1630-1631.1988
Abstract
We report here a new staining procedure which uses both the enzymatic dehydrogenation of 2-( p -iodophenyl)-3- p -(nitrophenyl)-5 phenyltetrazolium chloride to a pink intracellular formazan and the DNA-specific fluorochrome 4′,6′-diamidino-2-phenylindole. Application of this staining procedure to cells concentrated on filters and then transferred to microscope slides by the filter-transfer-freeze technique has proven valuable for statistically accurate enumeration of the total viable and metabolically active cells in groundwaters.This publication has 8 references indexed in Scilit:
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