The quantitative determination of dehydrogenase activity in cell suspensions

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Abstract
A colorimetric method was descr. for the quantitative detn. of dehydrogenase activity in yeast and other cell suspensions. To the cell suspension were added buffer, substrate and triphenyltetrazolium Br (TTB) and after incubation, it was acidifed, thus dissolving the formozan which was formed from TTB by reduction, which was then extracted with toluene and the color measured in a photoelectric absorptiometer. The method was considered to be very advantageous for measurements in the presence of activators or inhibitors.