Rapid Determination of Serum Zonisamide Concentration by a High-performance Liquid Chromatographic Method.
Open Access
- 1 January 1992
- journal article
- Published by Japanese Society of Pharmaceutical Health Care and Sciences in Japanese Journal of Hospital Pharmacy
- Vol. 18 (3) , 230-235
- https://doi.org/10.5649/jjphcs1975.18.230
Abstract
A simple HPLC method for the determination of zonisamide in serum for the pretreatment with methyl alcohol is reported.A reversed phase column (Yanapack ODS-A) was used and the mobile phase consisted of a mixture of 50 mM ammonium acetate and methyl alcohol (2: 1). Furosemide was used as an internal standard.Linear relationship between zonisamide levels in serum and peak area ratio (zonisamide/internal standard) was observed in the range of 1-40μg/ml.Ethosuximide peak was close to zonisamide peak.Simultaneous administration of ethosuximide and zonisamide was thought to be very rare, therefore, the serum level of zonisamide could be determined with this procedure.The serum zonisamide level of patients treated with zonisamide and other antiepileptics (phenytoin, carbamazepine, valproic acid) was lower than that of patients treated with monotherapy of zonisamide.Keywords
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