HPLC Measurement of Dansyl-Thyroxine in Femtomole Range

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Abstract
The formation of fluorophores by the action of dansyl chloride (1-dimethylaminonaphthalene-5-sulfonyl chloride) with iodoamino acids has provided a highly sensitive method for the detection of these compounds in the femtomole range. A relatively simple system is described for the reaction, chromatographic separation and characterization of individual iodoamino acids. Dansyl chloride reacts specifically with the primary amino group. The spectral characteristics of dansyl-thyroxine [T4] showed an excitation of 295 nm and an emission of 510 nm. The fluorescence of Dansyl-T4 in the femtomole range was linear with a coefficient of determination of 0.99.