Lac repressor - lac operator interaction. Circular dichroism study
Open Access
- 10 October 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 9 (19) , 5175-5184
- https://doi.org/10.1093/nar/9.19.5175
Abstract
The interaction between lac repressor and a small operator DNA fragment have been examined by circular dichroism spectroscopy. The binding of lac represser on the operator induces a conformational change of the DNA which is different from that observed upon non specific binding on non operator DNA. The CD titration curve indicates that the stoechiometry of interaction is complex. A two operators-one repressor complex was found. This result was confirmed by a gel filtration experiment.Keywords
This publication has 36 references indexed in Scilit:
- Interaction of lac repressor with alternating poly d (A-T) and poly d (G-C). Circular dichroism studiesBiochimie, 1980
- Correlations between the duplex winding angle and the circular dichroism spectrum of calf thymus DNABiochemistry, 1979
- Studies on gene control regionsJournal of Molecular Biology, 1978
- Cloning of chemically synthesized lactose operators II. EcoRI-linkered operatorsGene, 1978
- Molecular parameters characterizing the interaction of Escherichia coli lac repressor with non-operator DNA and inducerBiochemistry, 1977
- Construction, Isolation and Implications of Repressor‐galactosidase ·ß‐galactosidase Hybrid MoleculesEuropean Journal of Biochemistry, 1977
- Photochemical reactions of lac repressor. Effects on inducer bindingBiochemical and Biophysical Research Communications, 1977
- Lac repressor and Lac operatorProgress in Biophysics and Molecular Biology, 1976
- Lac repressor binding to poly [d(A-T)]. Conformational changesBiochemical and Biophysical Research Communications, 1974
- The ultraviolet circular dichroism of some natural DNAs and an analysis of the spectra for sequence informationBiopolymers, 1972