Nitrosomonas europaea Expresses a Nitric Oxide Reductase during Nitrification
- 1 July 2004
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 186 (13) , 4417-21
- https://doi.org/10.1128/jb.186.13.4417-4421.2004
Abstract
In this paper, we report the identification of a norCBQD gene cluster that encodes a functional nitric oxide reductase (Nor) in Nitrosomonas europaea . Disruption of the norB gene resulted in a strongly diminished nitric oxide (NO) consumption by cells and membrane protein fractions, which was restored by the introduction of an intact norCBQD gene cluster in trans . NorB-deficient cells produced amounts of nitrous oxide (N 2 O) equal to that of wild-type cells. NorCB-dependent activity was present during aerobic growth and was not affected by the inactivation of the putative fnr gene. The findings demonstrate the presence of an alternative site of N 2 O production in N. europaea .Keywords
This publication has 24 references indexed in Scilit:
- Complete Genome Sequence of the Ammonia-Oxidizing Bacterium and Obligate Chemolithoautotroph Nitrosomonas europaeaJournal of Bacteriology, 2003
- Nitrite Reductase of Nitrosomonas europaea Is Not Essential for Production of Gaseous Nitrogen Oxides and Confers Tolerance to NitriteJournal of Bacteriology, 2002
- Spectroscopic Characterization of the NO Adduct of Hydroxylamine OxidoreductaseBiochemistry, 2002
- FnrP and NNR of Paracoccus denitrificans are both members of the FNR family of transcriptional activators but have distinct roles in respiratory adaptation in response to oxygen limitationMolecular Microbiology, 1997
- Purification and initial kinetic and spectroscopic characterization of NO reductase from Paracoccus denitrificansBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1997
- Mutational Analysis of the Nor Gene Cluster which Encodes Nitric‐Oxide Reductase from Paracoccus denitrificansEuropean Journal of Biochemistry, 1996
- DNA Binding and Dimerization of the Fe−S-containing FNR Protein from Escherichia coli Are Regulated by OxygenJournal of Biological Chemistry, 1996
- Isolation and Characterization of a Novel Insertion Sequence Element, IS1248, in Paracoccus denitrificansPlasmid, 1995
- Production of nitric oxide in Nitrosomonas europaea by reduction of nitriteArchiv für Mikrobiologie, 1990
- A nitrite-reducing enzyme from Nitrosomonas europaea Preliminary characterization with hydroxylamine as electron donorBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1968