Immature maize spikelets develop and produce pollen in culture
- 1 June 1992
- journal article
- Published by Springer Nature in Plant Cell Reports
- Vol. 11 (5)
- https://doi.org/10.1007/bf00235075
Abstract
Immature maize spikelets have been successfully grown in vitro. Culture conditions were refined to maximize development of normal pollen grains. Kinetin was not required for normal development, in contrast to the absolute requirement for this plant growth regulator for in vitro tassel development. Development occured in all stages sampled, from premeiosis to postvacuolation, and there was no lag in progression through the various stages of development as compared to greenhouse-grown material. Cultured spikelets produced pollen that appeared morphologically normal, accumulated starch and had the normal two sperm nuclei and single vegetative nucleus.Keywords
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