Delayed Hypersensitivity, as Manifested by Granuloma Formation, Dermal Reactivity, Macrophage Migration Inhibition and Lymphocyte Transformation, Induced and Elicited in Guinea Pigs with Soluble Antigens of Schistosoma Mansoni Eggs

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Abstract
Delayed hypersensitivity (DH) was induced in guinea pigs 3 to 6 days after immunization with microgram quantities of soluble antigens derived from Schistosoma mansoni eggs (SEA). Sensitization was achieved by incorporating SEA into complete Freund's adjuvant, but remarkably, animals could also be sensitized by intraperitoneally injected live eggs, or by direct injection of SEA alone into the footpad. DH was measured by comparing dermal and granulomatous reactivity in vivo and 3H-thymidine incorporation and macrophage migration inhibition (MI) in vitro. SEA could be employed successfully also for the elicitation of the different immune responses. The parameters appeared in parallel, MI preceding the rest by 1 day. Granuloma formation, which occurred within 24 hr in the lungs of sensitized animals, correlated well with other parameters of DH. During the 1st 10 days of immunization, when DH was already fully developed, γ1 or γ2 antibodies could not be detected in the circulation of immunized animals.

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