Ultrastructural cytochemistry of the ocular lens

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Abstract
Ultrastructural and cytochemical investigations of ocular lens are difficult due to limited applicability of currently available techniques for the preparation of this tissue for such studies. Several modifications in currently available techniques are being made in order to preserve the fine structure of the lens. A modified method is presented which provides good preservation of [rat] lens fine structure and allows cytochemical localization of enzymes in this tissue. Brief freezing permits preparation of thin slices of the lens which are subsequently incubated by the appropriate media for enzyme cytochemistry at an ultrastructural level. This modified method was used for the localization of Na+-K+-dependent ATPase.