The effect of divalent cations on the rosette formation via C3 receptors.

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Abstract
The indicator cells with the specificity for the various C3 [complement component 3] receptors were prepared using highly purified complement components. The effect of EDTA and divalent cations on the rosette formation of these indicators with human blood cells and lymphoblastoid cells were investigated. B lymphocytes form rosettes with EACl-3b [erythrocyte-antibody-C1-C3b], EAC1-3bH [EAC1-3b-Factor H] EAC1-3bi and EAC1-3d irrespective of the presence of cations. PMN [polymorphonuclear leukocyte] and monocytes form rosettes with EAC1-3b and EAC1-3bH independent of cations, but their formation with EAC1-3bi needs the presence of Mg. In this case Ca shows a cooperative effect with Mg. PMN and monocytes do not react with EAC1-3d even in the presence of Mg. Although Raji cells do not react with EAC1-3b, they react with EAC1-3bH independent of divalent cations. EACm has a similar reactivity to EAC1-3bi and by the treatment with trypsin it becomes similar to EAC1-3d.