Cloning of genes developmentally regulated during plant embryogenesis

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Abstract
Genes specifically induced during somatic embryogenesis may play key roles in plant embryo development. An antiserum against an extract or carrot somatic embryos revealed a few rare antigens induced at the onset of embryogenesis. Through differential immunoadsorption techniques, we purified antibodies against the embryo-specific antigens and probe a phage .lambda. gt11 library of cDNA from carrot somatic embryos. This paper describes three distinct cDNA clones that hybridize to embryo-specific RNAs. Monospecific antibodies, purified by affinity to the recombinant phage fusion proteins, confirm that the cloned cDNAs encode unique embryo-specific peptide antigen. One 50-kDa protein correlates with embryogenic ability in cultures of other plant species, including cereals.