Global Changes in Gene Expression in Sinorhizobium meliloti 1021 under Microoxic and Symbiotic Conditions
Open Access
- 1 March 2004
- journal article
- research article
- Published by Scientific Societies in Molecular Plant-Microbe Interactions®
- Vol. 17 (3) , 292-303
- https://doi.org/10.1094/mpmi.2004.17.3.292
Abstract
Sinorhizobium meliloti is an α-proteobacterium that alternates between a free-living phase in bulk soil or in the rhizosphere of plants and a symbiotic phase within the host plant cells, where the bacteria ultimately differentiate into nitrogen-fixing organelle-like cells, called bacteroids. As a step toward understanding the physiology of S. meliloti in its free-living and symbiotic forms and the transition between the two, gene expression profiles were determined under two sets of biological conditions: growth under oxic versus microoxic conditions, and in free-living versus symbiotic state. Data acquisition was based on both macro- and microarrays. Transcriptome profiles highlighted a profound modification of gene expression during bacteroid differentiation, with 16% of genes being altered. The data are consistent with an overall slow down of bacteroid metabolism during adaptation to symbiotic life and acquisition of nitrogen fixation capability. A large number of genes of unknown function, including p...Keywords
This publication has 67 references indexed in Scilit:
- Mutations insit Bandsit Dgenes affect manganese-growth requirements inSinorhizobium melilotiFEMS Microbiology Letters, 2003
- The FixM Flavoprotein Modulates Inhibition by AICAR or 5′AMP of Respiratory and Nitrogen Fixation Gene Expression in Sinorhizobium melilotiMolecular Plant-Microbe Interactions®, 2002
- A Sinorhizobium meliloti Lipopolysaccharide Mutant Induces Effective Nodules on the Host Plant Medicago sativa (Alfalfa) but Fails to Establish a Symbiosis with Medicago truncatulaMolecular Plant-Microbe Interactions®, 1998
- Identification and Characterization of a Gene on Rhizobium meliloti pSyma, syrB, That Negatively Affects syrM ExpressionMolecular Plant-Microbe Interactions®, 1997
- Properties of NAD+- and NADP+-dependent malic enzymes of Rhizobium (Sinorhizobium) meliloti and differential expression of their genes in nitrogen-fixing bacteroidsMicrobiology, 1997
- Characterization of aRhizobium melilotiProline Dehydrogenase Mutant Altered in Nodulation Efficiency and Competitiveness on Alfalfa RootsMolecular Plant-Microbe Interactions®, 1995
- Outer Membrane Protein Changes During Bacteroid Development Are Independent of Nitrogen Fixation and Differ Between Indeterminate and Determinate Nodulating Host Plants ofRhizobium leguminosarumMolecular Plant-Microbe Interactions®, 1995
- A novel eubacterial phylum: comparative nucleotide sequence analysis of atuf-gene ofFlexistipes sinusarabiciFEMS Microbiology Letters, 1991
- Mechanism of Nitrogenase Inhibition in Soybean NodulesPlant Physiology, 1990
- R Factor Transfer in Rhizobium leguminosarumMicrobiology, 1974