Effect of estrogen on preprolactin messenger ribonucleic acid sequences
- 1 May 1979
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 18 (10) , 2044-2048
- https://doi.org/10.1021/bi00577a031
Abstract
A complementary DNA (cDNA) probe was synthesized for rat preprolactin mRNA. Following preparative gel electrophoresis, the cDNA was characterized and estimated to be .apprx. 75% pure. The reverse transcript was estimated to be greater than 1000 nucleotides long and, therefore, was a full-length copy of the preprolactin mRNA. It was a faithful transcript, as evidenced by back-hybridization with the mRNA. By use of the cDNA as a hybridization probe, the levels of preprolactin mRNA in the pituitary of male and female rats were monitored and found to increase following estrogen treatment. Increases in preprolactin mRNA activity, assayed by in vitro translation in the wheat germ system, were paralleled by increases in hybridization to the cDNA probe after estrogen treatment.This publication has 3 references indexed in Scilit:
- Importance of full size complementary DNA in nucleic acid hybridization.Journal of Biological Chemistry, 1976
- Ovalbumin Messenger RNA: Evidence That the Initial Product of Transcription Is the Same Size as Polysomal Ovalbumin MessengerProceedings of the National Academy of Sciences, 1974
- Sedimentation studies of the size and shape of DNAJournal of Molecular Biology, 1965