Further Studies on the Fractionation of Ribonuclease I-Core by Gel Filtration

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Abstract
Yeast RNA was digested by pancreatic RNAse I, the core passed through a Sephadex gel (G-25, G-50 or G-75) column. Fractions I and II collected in the order of elution, showed sedimentation constants of 3.5 and 0.4 S, respectively and an average chain length of 7.1 and 5.5, by the phospho-monoesterase digestion method. The Streptolysin S[image]-forming potency was found only in Fraction L RNAse I-core of deaminated yeast RNA, RNAse T1- and Bacillus subtilis RNAse-cores of yeast RNA could not be separated by Sephadex gel filtration. The RNAse I-core was also separated by an ECTELA-cellulose column to the 0.3 [image] and 0.5 [image] NaCl-eluted fractions, and the latter was further separated into 2 sub-fractions by Sephadex gel, G-75, filtration, one of which was enriched in Streptolysin S[image]-forming potency.
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