Some observations on the zinc in carbonic anhydrase

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Abstract
Carbonic anhydrase kept as long as 32 days at 0[degree] and pH 7.4 in a solution of Zn65-containing Zn salts (plus glycine to prevent precipitation of the Zn) took up no significant amount of Zn65. When red blood cells were labelled in vitro with Zn65, little or none of the Zn which entered the red cell was firmly attached to the carbonic anhydrase. The inability of carbonic anhydrase to exchange its Zn with Zn ions and simple Zn compounds supported the view of Keilin and Mann that the metal which was an integral part of the enzyme molecule was firmly bound to the protein.