Contact sensitivity to oxazolone in the mouse. VIII. Demonstration of several classes of antibody in the sera of contact sensitized and unimmunized mice by a simplified antiglobulin assay.

  • 1 September 1972
    • journal article
    • Vol. 23  (3) , 289-98
Abstract
CBA mouse antibodies to oxazolone were assayed by direct and antiglobulin augmented haemagglutination, using oxazolone coupled sheep erythrocytes. The antiglobulin Coombs' test was simplified by eliminating washing the sensitized cells and running the entire assay in a microtitre plate. Oxazolone antibodies of IgM, IgG1 and IgG2a classes were found in unimmunized mice, and antibodies of significantly higher titre were found in IgM, IgG1, IgG2a, and IgG2b classes of contact sensitized mice. Because the demonstration of oxazolone antibodies in the sera of unimmunized mice required highly oxazolonated SRBC, it was suggested that these antibodies are of relatively low binding avidity.