The synthesis of oligonucleotides containing a primary amino group at the 5'-terminus
Open Access
- 10 April 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (7) , 3131-3139
- https://doi.org/10.1093/nar/15.7.3131
Abstract
Oligonucleotides containing a primary amino group at their 5'-termini have been prepared and further derivatised with amino specific probes. The sequence required is prepared using standard solid phase phosphoramidite techniques and an extra round of synthesis is then performed with N- onomethoxytrityl—0-methoxydiisopropylaminophosphinyl 3-aminopropan(1)ol. After cleavage from the resin, removal of the phosphate and base protecting groups and purification gives a monomethoxytrityl-NH(CH2)3PO4, -oligomer. The monomethoxytrityl group can be removed with acetic acid to give the desired amino containing oligomer. The amino group can be further derivatised with amino specific probes yielding fluorescent or biotinylated oligonucleotide products.Keywords
This publication has 5 references indexed in Scilit:
- Efficient methods for attaching non-radioactive labels to the 5′ ends of synthetic oligodeoxyribonucleotidesNucleic Acids Research, 1986
- The synthesis of oligonucleotides containing an aliphatic amino group at the 5′ terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysisNucleic Acids Research, 1985
- Chemical synthesis of oligonucleotides containing a free sulphydryl group and subsequent attachment of thiol specific probesNucleic Acids Research, 1985
- Polymer support oligonucleotide synthesis XVIII1.2): use ofβ-cyanoethyi-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final productNucleic Acids Research, 1984
- The synthesis of glycyl-l-phenylalanyl-sRNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967