Relationship of phosphorylation to the oligomerization of SV40 T antigen and its association with p53
- 28 January 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 180 (2) , 285-290
- https://doi.org/10.1016/0014-5793(85)81087-5
Abstract
The potential significance of the phosphorylation of SV40 large T antigen for oligomers and complexes with the cellular protein p53 was investigated. We observed that T antigen oligomers remain stable after enzymatic dephosphorylation by alkaline phosphatase up to 80%. Separate analysis of free and p53‐bound T antigen revealed a considerably lower phosphorylation of the p53‐bound subclass. Therefore, a simple correlation between the overall phosphorylation of T antigen and the formation of oligomers and T‐p53 complexes is highly unlikely.Keywords
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