REDUCTION OF TUMOR BURDEN IN A MURINE OSTEO-SARCOMA FOLLOWING HYPERTHERMIA COMBINED WITH CYCLOPHOSPHAMIDE

  • 1 January 1984
    • journal article
    • research article
    • Vol. 44  (4) , 1405-1408
Abstract
A radiation- and chemotherapy-resistant murine osteosarcoma was used to investigate the effect of local hyperthermia (42.5 .+-. 0.1.degree., 30 min) alone and in combination with cyclophosphamide. The cytotoxicity of cyclophosphamide on murine osteosarcoma was established previously. Local hyperthermia (42.5 .+-. 0.1.degree., 30 min) had little or no effect on the 16-day-old (206 .times. 106 osteosarcoma tumor cells/mouse) tumor as shown by the changes in the tumor cell marker, alkaline phosphatase. A 2.5 .+-. 3.5% reduction in the number of tumor cells was seen. Large tumors treated at 21 days postimplantation (357 .times. 106 tumor cells) showed a reduction of 24 .+-. 14%. The effect of combination treatment with cyclophosphamide and hyperthermia produced greater reduction in the numbers of tumor cells than did either treatment used alone.